Joint work in Valencia

Jelena and Ilija spent two weeks in Valencia (Spain) working at the Universitat Politècnica de València in the group for Aquaculture and Biodiversity leading by Juan F. Asturiano. It was continuing of long-term fruitful collaboration of our two groups. The main topic of our visit was developing protocols for cryopreservation of eel gonads. All experiments were successful and we are looking forward for the next step in our joint research. We greatly appreciate your warm hospitality, thank you for everything Johnny, German and Victor. 

Eye to eye

Ilija and Victor in a hunting mode

Dissection

Pippeting, filtering....with German

Preparation for viability test

Vitrification

Ákos was there to help in the critical moment

Hunting mode vol.2

Checking viability of the cells

Course "Fluorescence microscopy - FluoMicro@ICGEB"

Ilija participated at the Course "Fluorescence microscopy - FluoMicro@ICGEB" organized by International Centre for Genetic Engineering and Biotechnology - ICGEB in Trieste, Italy, in the period 2-4 October 2018. During the course, following topics we covered:

• Confocal Imaging
• High-throughput imaging
• Time-lapse microscopy
• Image processing and quantitative analysis

New paper in Theriogenology

A new paper in collaboration with our colleagues from Kaposvár University (Hungary) has been accepted for publication in Theriogenology.

 

Müller T., Szabó T., Kollár T., Csorbai B., Marinović Z., Horváth L., Kucska B., Bodnár Á., Urbányi B., Horváth Á. 2018. Artificial insemination of African catfish (Clarias gariepinus) using cryopreserved sperm. Theriogenology. In press. DOI: 10.1016/j.theriogenology.2018.09.034

 A B S T R A C T
In this study, we aimed to develop a practical protocol for using cryopreserved sperm for induced/wild/tank spawning of fish species with external fertilization. Experiments were carried out on African catfish (Clarias gariepinus) as a model species. Sperm was collected for cryopreservation and diluted with the cryomedium (266mM fructose, 20% methanol) at a ratio of 1:1 with a final methanol concentration of 2.47M pH7.73. Diluted sperm was loaded into 0.5-ml straws and cryopreserved by conventional protocol. Samples were prepared for insemination 24 h later, by thawing for 13 s in a 40°C water bath, and centrifuged at 500×g for 10minat 20°C. The seminal plasma, extender and external cryoprotectant were removed from the concentrated spermatozoa. The pellet was then resuspended in common carp (Cyprinus carpio) seminal plasma to reconstitute the lost volume. Sperm samples were then injected by a catheter into the ovarian cavity through the oviduct of the experimental females by the so-called ovarian lavage method in parallel with the intramuscular hormonal administration (5mg carp pituitary/kg bw). Inseminated females (n=9) were monitored for 10h and ovulated eggs and spermatozoa stored in in the ovary were stripped. Stripped gamete samples were divided into two batches: (1) the first batch contained only the previously injected spermatozoa and was activated by aerated water (WA) immediately after stripping; (2) in case of the second batch additional, freshly stripped sperm was added as positive control to the stripped eggs before water activation (PC). Furthermore, five females were propagated by using the dry fertilization method (in vitro fertilization) as negative control (NC). All sperm and hormone injected females produced fertilised eggs with a hatching rate of 17.7±13.2%, 12.5±9.3%, and 61±11.5% for WA, PC and NC respectively. These results indicate that artificial insemination based on using cryopreserved sperm with ovarian lavage can be a viable alternative to in vitro fertilization in a catfish species. Thus, we describe a proof of principle for a practical protocol for the induced/wild/tank spawning of an externally fertilising fish species with economical importance and propose that the protocol could be also applied to endangered marine or fresh fish species.

The second meeting of biologists in Serbia

Jelena, Ilija and Zoran have participated at The second meeting of biologists in Serbia organized in Kladovo, Serbia (25th – 30th September). During the meeting, they gave two oral and two poster presentations of papers done in collaboration with our colleagues from Japan, Slovenia and Serbia.

Lujić J., Marinović Z., Sušnik Bajec S., Kása E., Urbányi B., Horváth Á. 2018. Cryopreservation and transplantation of germ cells as a method for ichthyofauna conservation. [in Serbian]. Abstract book. The second Congress of Biologist in Serbia; 25-30 Sepember, 2018, Kladovo, Serbia.pp.92.

Šćekić I., Marinović Z., Lujić J., Kása E., Kollár T., Urbányi B., Horváth Á. 2018. Identification of sperm subpopulations of carp sperm [in Serbian]. Abstract book. The second Congress of Biologist in Serbia; 25-30 Sepember, 2018, Kladovo, Serbia.pp.292.

Marinović Z., Lujić J., Yoshizaki G., Li Q., Garai E., Csenki Zs., Urbányi B., Horváth Á. 2018. Conservation of different zebrafish lines by spermatogonia transplantation. [in Serbian]. Abstract book. The second Congress of Biologist in Serbia; 25-30 Sepember, 2018, Kladovo, Serbia.pp.315.

Marinović Z., Kostić D., Marković G., Bolić-Trivunović V., Miljanović B., Lujić J. 2018. Growth of gibel carp Carassius gibelio (Bloch, 1782): adventages of multimodel inference. [in Serbian]. Abstract book. The second Congress of Biologist in Serbia; 25-30 Sepember, 2018, Kladovo, Serbia. pp.120.    

 

Ready

Biologists and friends

Ilija presenting

Poster session

Chairman and chairwoman :)

Jelena presenting

Beautiful Kladovo

Danube in the National park Djerdap