6/24/2022

Conference in Croatia

 On June 19-24th, Nevena, Betti and Ákos participated at the 57th Croatian and 17th International Symposium of Agriculture in Vodice, Croatia. We were accompanied by our colleagues Dr. Balázs Kovács of the Department of Molecular Ecology, Jelena Stanivuk, PhD student under the joint supervision of Ákos and Dr. Uroš Ljubobratović working at the Research Center of Fisheries and Aquaculture (HAKI), Dr. Mária Kovács-Weber of the Institute of Animal Husbandry Sciences and Dr. István Lehoczky of the National Biodiversity and Gene Conservation Center. A
s usual, the conference had a session on Fiheries, Game Management and  Beekeeping with Ákos co-chairing a part of the morning session on Fisheries together with Dr. Zvjezdana Popović Perković of the University of Split. The conference was an excellent opportunity to strengthen our collaboration with Croatian colleagues and to become acquainted with each other’s work.

The works presented by the members of our team:

Ákos Horváth, Bernadett Pataki, Zoran Marinović, Béla Urbányi: Inherited cryoresistance of fish sperm: is it real? 

Nevena Kitanović, Zoran Marinović, Bernadett Pataki, Balázs Csorbai, Gergely Mészáros, Ákos Horváth: Optimization of Leibovitz L-15 media for in vitro maturation of common carp ovarian follicles 

Bernadett Pataki, Ádám Staszny, Gergely Mészáros, Nevena Kitanović, András Ács, Árpád Hegyi, József Molnár, Balázs Csorbai, Béla Urbányi, Ákos Horváth: Morphological changes in common carp (Cyprinus carpio) progeny induced by the use of cryopreserved sperm 

Jelena Stanivuk , Zoran Marinović, Uroš Ljubobratović, Ákos Horváth: Fixation of histological specimens of juvenile testicular tissue of pikeperch (Sander lucioperca) and Eurasian perch (Perca fluviatilis


Ákos giving his presentation

Nevena giving her presentation

Betti presenting her results

Ákos co-chairing the session with Dr. Zvjezdana Popović Perković 

The team after the session (photo by O. Barić)





5/17/2022

Another paper published in Aquacultrue Reports

 A new paper has been published in the journal Aquaculture Reports in collaboration with our colleagues from the Department of Animal Science, Biotechnical Faculty, University of Ljubljana and the Lillafüred Trout Farm in Hungary. Enjoy!

Marinović, Z., Lujić, J., Sušnik Bajec, S., Djurdjevič, I., Snoj, A., Hoitsy, G., Urbányi, B., Horváth, Á., 2022. Evaluation of triploid rainbow trout Oncorhynchus mykiss as a surrogate parent for brown trout Salmo trutta m. fario and grayling Thymallus thymallus. Aquac. Reports 24, 101163. https://doi.org/10.1016/j.aqrep.2022.101163

Abstract:

Surrogate production through germline stem cell (GSC) transplantation is becoming a very promising technology in species management and ex situ species conservation. In the present study, we have evaluated if the triploid rainbow trout is a suitable surrogate parent for the production of other salmonid gametes. In three independent trials conducted in two countries, we have transplanted brown trout and grayling GSCs into triploid rainbow trout larvae as recipients. Two months after transplantation, dissection of recipients displayed that both spermatogonial (SSCs) and oogonial (OSCs) stem cells of both species were able to colonize recipient gonads. After three years of rearing, neither male nor female recipients displayed signs of gametogenesis progression. Species-specific (for both donor and recipient species) amplification of mtDNA control region on the other hand displayed a presence of donor-derived germ cells within recipient gonads. This indicated that even though donor cells were able to colonize recipient gonads, they remained in a dormant or quasi-dormant state, and did not progress with gametogenesis. This study displays that the rainbow trout is not a suitable recipient for all salmonid species, and that careful selection of recipients is a crucial step in developing the surrogate production technology.

Work in Croatia

Within the frames of the Hungarian-Croatian bilateral project, between May 9-13th we have enjoyed the hospitality of Ana Gavrilović, her team from the University of Zagreb as well as the staff of the Aquarium Pula in Pula, Croatia. The goal of the project is to develop cryopreservation methods for the gametes and larvae of various bivalvian species found in the Adriatic sea including the European flat oyster (Ostrea edulis), the mediterranean scallop (Pecten jacobaeus) and possibly for the critically endangered noble pen shell (Pinna nobilis) whose populations have been severely affected by the pathogen Haplosporidium pinnae. Ákos and Zoran were accompanied by Dr. Balázs Kovács and Réka Balogh of the Department of Molecular Ecology of our institute who plan to carry out molecular studies on the species. We wish to thank all our Croatian friends and colleagues for their hospitality and help! Within the frames of the Hungarian-Croatian bilateral project, between May 9-13th we have enjoyed the hospitality of Ana Gavrilović, her team from the University of Zagreb as well as the staff of the Aquarium Pula in Pula, Croatia. The goal of the project is to develop cryopreservation methods for the gametes and larvae of various bivalvian species found in the Adriatic sea including the European flat oyster (Ostrea edulis), the mediterranean scallop (Pecten jacobaeus) and possibly for the critically endangered noble pen shell (Pinna nobilis) whose populations have been severely affected by the pathogen Haplosporidium pinnae. Ákos and Zoran were accompanied by Dr. Balázs Kovács and Réka Balogh of the Department of Molecular Ecology of our institute who plan to carry out molecular studies on the species. We wish to thank all our Croatian friends and colleagues for their hospitality and help!


Zoran and Ákos demostrating the two-pipette dilution technique

Réka and Balázs taking samples from oyster for molecular analysis


Balázs and Zoran analyzing the survival of cryopreserved oyster larvae

The team in front of the famous Arch of Sergii in Pula



5/11/2022

Preparing for Aquaculture Europe 2023

 Last week, Ákos and Professor Béla Urbányi, director of the Institute of Aquaculture and Environmental Safety has attended the meeting hosted by the European Aquaculture Society (EAS) to initiate preparation for the most important annual EAS event, the Aquaculture Europe conference in 2023. The meeting will be held in Vienna, Austria and is scheduled for September, 2023. The main theme of the meeting will be balanced diversity in aquaculture development.

As Vienna lies in the heart of Central Europe, the preparatory activities also involved the participation of several parties, including colleagues from Austria, Hungary, Czech Republic and Germany. Participants discussed various issues related to the organization, including the venue, program, conference sessions, student involvement and distribution of tasks to various committees. Béla has assumed the role of program co-chair of the meeting while Ákos is a member of the Local Organizing Committee charged with publicizing the event on various platforms. 

We would like to thank Alistair Lane, Mario Stael and John Cooksey for the organization and we are looking forward to our continued collaboration.



4/20/2022

Return to Slovenia

After a 5-year hiatus, we finally were able to return to beautiful Slovenia! Thanks to the invitation by our colleague, Dr. Simona Sušnik Bajec of the Department of Animal Science, Biotechnical Faculty, University of Ljubljana we were able to collaborate with her and her PhD student Tjaša Kodela on the reproduction of the grayling (Thymallus thymallus). Betti and Ákos spent 3 days working at the hatchery of the Fisheries Research Institute of Slovenia in Kobarid, stripping sperm and eggs and checking sperm quality using CASA. Grayling spawning has drastically reduced during the last decade in the Soča river and the Institute puts a considerable effort into the culture and repopulation of this fish species. Hopefully, we will be able to assist Tjaša in her scientific studies.


Grayling males waiting to be stripped

Ákos and Betti in the hatchery

Ákos and Tjaša at work

4/01/2022

New paper published in Aquaculture Reports

A new paper with Betti's first authorship has been published in Aquaculture Reports. Enjoy: 

Bernadett Pataki, Ákos Horváth, Gergely Mészáros, Nevena Kitanović, András Ács, Árpád Hegyi, József Molnár, Balázs Csorbai, Béla Urbányi. 2022. Adjustment of common carp sperm concentration prior to cryopreservation: Does it matter? Aquaculture Reports Volume 24, June 2022, 101109 https://doi.org/10.1016/j.aqrep.2022.101109 

Abstract 

Absorbance and computer-assisted sperm analysis (CASA) was used to assess the sperm concentration of common carp (Cyprinus carpio) as an alternative to hemocytometer counting. Also the effect of different sperm concentrations on post-thaw sperm parameters and fertilizing capacity were tested. The main goal of the research was to find out whether the tested methods are suitable for fast measurement of sperm concentration and if the pre-setting of sperm concentration before cryopreservation offers any advantages over dilution of sperm to a given ratio. A linear relationship of sperm concentration measured with CASA (N = 12) as well as sperm absorbance assessed with a microplate reader with spectrophotometry module (N = 18) with sperm concentration counted in a Bürker-Türk type hemocytometer was established. For cryopreservation, 4 sperm concentrations were used (0.5; 1; 2; 4 × 109 spermatozoa per ml) and compared to dilution to a ratio of 1:9. A significant positive relationship was found between the absorbance and sperm concentration (p < 0.0001, r2 = 0.8289) as well as between immotile cell concentration measured with CASA and that counted in a hemocytometer (p < 0.0001, r2 = 0.8559). No significant main effect of sperm concentration was found on any post-thaw motility parameters measured by CASA with the exception of linearity (p = 0.0056). Sperm concentration had a significant main effect (p = 0.0156) on the fertilizing capacity of sperm samples diluted to various concentrations prior to cryopreservation. Only the highest concentration of 4 × 109 spermatozoa per ml (66 ± 6% fertilization) showed a significant difference (p = 0.0121) in comparison to the pre-set dilution ratio (49 ± 5% fertilization). The results show that both spectrophotometry and CASA are suitable for the assessment of common carp sperm concentration and that maximizing sperm concentration prior to cryopreservation results in higher fertilization.

11/28/2021

Training of Marta Blanes and Leonor Ferrão

 Between October 24th and November 26th, this year we had the pleasure of hosting Marta Blanes and Leonor Ferrão of the Group of Aquaculture and Biodiversity under the leadership of Prof. Juan F. Asturiano at the Polytechnical University of Valencia in Spain. Marta and Leonor were trained by Zoran in various techniques including isolation of germline stem cells (GSCs), treatment of embryos with  morpholinos to induce sterility as well as transplantation of GSCs into suitable recipents. Their work was part of the EELGONIA project funded by the Spanish Ministry of Science and Innovation. During their stay, Marta and Leonor have isolated GSCs from cryopreserved gonads of the European eel and transplanted them under Zoran's supervision into sterilized zebrafish and common carp larvae. The recipient larvae are currently grown in our facilities and we cannot wait to see what will happen to the transplanted cells.

Marta and Leonor with members of our team in front of our building

11/20/2021

Aquaculture Europe 2021

 Zoran and Ákos attended the Aquaculture Europe 2021 meeting that was held on October 4-7 in Funchal, the capital city of the beautiful island of Madeira in Portugal. The meeting was organized by the European Aquaculture Society and attended by more than 1400 participants. The conference was of special interest for us because of the opportunity to meet our friends Juan F. Asturian of the Polytechnical University of Valencia (Spain) and Elsa Cabrita from CCMAR and University of Algarve (Portugal) who was also co-chairing the session on Reproduction. The conference was very well-organized with high-quality sessions and a large trade show. Regarding the cons, the e-poster session was virtually invisible and completely insignificant. A classical poster session with poster boards would have been much nicer. Nevertheless, we had a good time and will definitely come to EAS next year.

The posters presented were:

Á. Horváth, B. Pataki, G. Mészáros, Z. Marinović, N. Kitanović, B. Urbányi: Does sperm concentration matter in carp sperm cryopreservation?

Z. Marinović, J. Lujić, S. Sušnik Bajec, I. Djurdjevič, A. Snoj, B. Urbányi and Á. Horváth: Triploid rainbow trout Oncorhynchus mykiss as a recipient of brown trout germline stem cells.

Bernadett Pataki, Béla Urbányi, Tímea Kollár, Ákos Horváth: Inheritance of sperm cryoresistance in common carp (Cyprinus carpio)

Ákos with Elsa Cabrita (CCMAR, Faro, Portugal) and Juan F. Asturiano (Universitat Politècnica de València, Spain)


9/13/2021

Conference in Croatia

Ákos and Betti along with our colleagues Dr. Balázs Kovács and Réka Balogh from the Department of Molecular Ecology have participated at the 56th Croatian and 16th International Symposium of Agriculture which was held between September 5-10th in Vodice, Croatia. Both Betti and Ákos presented the results of the group in several talks during the session on Fisheries, Game Management and Beekeeping. 

Prior to the meeting we also had the pleasure to visit the hatchery of the largest Croatian marine aquaculture company, Cromaris which is situated in the little coastal town of Nin. We had a guided tour of the hatchery by chief technologist Adelko Mihovilović and had the fortune of seeing a large-scale marine fish hatchery for the first time.

Visit to the hatchery of the marine aquaculture company Cromaris in Nin. Left to right: Ákos, Betti, chief technologist Adelko Mihovilović, Réka Balogh and Dr. Balázs Kovács

Betti giving her presentation

Ákos giving his presentation






7/11/2021

Zoran's defense

 The long anticipated day has finally come: one of the core members of our team, Zoran Marinović has defended his PhD title. His dissertation was entitled: "Intra- and inter-specific transplantation of spermatogonial stem cells in freshwater fish". The defense was held online on 5th July, 2021 and it was a special pleasure to host Dr. Elsa Cabrita of CCMAR/University of Algarve from Faro, Portugal in Gödöllő as one of Zoran's reviewers. This was the first time in the history of the PhD school of Animal Biotechnology and Animal Sciences that a foreign reviewer has taken part in the work of the defense committee. The committee has evaluated Zoran's presentation, dissertation and debate to a score of 100%. We congatulate Zoran to his success and wish him all the best. 

Well done, Dr. Marinović!

The committee during Zoran's online defense



After a job well done a dinner in Budapest on the banks of the Danube


Ági's defense

 The alumna of our group, Ágnes Ősz has successfully defended her PhD thesis on 28th May, 2021. Her dissertation was entitled "Genetic analysis of Hungarian brown trout populations and development of a marker-assisted breeding system". Ági gave an impressive performance and the committee evaluated her presentation and discussion to a score of 97%. We wish Ági all the best and congratulate her to her new title.

Good job, Dr. Ősz!

6/23/2021

New paper published in Aquaculture

 A new paper describing the genetic diversity of brown trout broodstocks throughout Europe was published with Ákos as a coauthor. The main conclusion of the paper is that there exists an international strain of Atlantic lineage of the species that is distributed throughout the continent that could potentially contribute to the reduction of the genetic diversity in brown trout. Enjoy!

Patrick Berrebi, Ákos Horváth, Andrea Splendiani, Stefan Palm, Rafał Bernaś (2021) Genetic diversity of domestic brown trout stocks in Europe. Aquaculture, Volume 544, 15 November 2021, 737043

Abstract

Brown trout (Salmo trutta) is composed of numerous geographical forms in the wild and a multitude of stocks reared in hatcheries. Practices impacting this species are domestication and fish farming. Thousands of hatcheries are producing domestic trout which are frequently released in the wild without real knowledge of the origin and genetic composition of the strains and of the receiving populations. The present study contains an analysis of the genetic structure (using twelve microsatellites) of 26 hatchery strains from Europe (Sweden, Poland, Czech Republic, Slovakia, Hungary, France, Italy and Spain) and one from North America (Minnesota). Several new observations improved our knowledge on this domestic part of S. trutta. First, a cross-border commercial strain of the North Atlantic lineage occupies most of European hatcheries and the American one, as a probable consequence of intensive exchanges of broodstock materials over time. In addition to the common Europe-wide commercial Atlantic strain, local strains stemming from domesticated regional wild fish also occur. Second, the level of genetic polymorphism of most hatchery strains is high to very high, likely reflecting genetic admixture counteracting expected losses of diversity through random genetic drift and domestication. This study emphasizes the value of identifying the genetic composition of hatchery stocks used for releases. It further stresses the need for caution when stocking a common stock across the whole geographical range of a species, with risks for reducing the intraspecific genetic diversity and local adaptation.

3/26/2021

Participation in an online workshop

Members of our team (Ákos, Nevena and Zoran) are participating in the 4th edition of the Workshop on Reproductive Biotechnology and Cryobanking in Aquatic Species organized by our colleagues from the Center of Marine Sciences (CCMAR) of the University of Algarve in Faro, Portugal. Ákos will give a talk on the principles of cryopreservation, and he will be joined by Nevena and Zoran in a video presentation of zebrafish gonad vitrification as well as transplantation of cells into suitable recipients. Registration is free and open until April 2nd



2/06/2021

Institutional changes



As of February 1st, 2021 Szent István University no longer exists. Following a merger of SZIU with the University of Kaposvár, the Georgikon Faculty of Agriculture of the University of Pannonia and the Károly Róbert Campus of Eszterházy University on August 1st, 2020, a new merger took place last Monday when the National Agricultural Research and Innovation Centre (NAIK) was integrated into the university structure. 

The new university is called Hungarian University of Agriculture and Life Sciences (Magyar Agrár- és Élettudományi Egyetem – MATE). The university has five campuses: Buda (Budapest), Szent István (Gödöllő), Georgikon (Keszthely), Kaposvár (Kaposvár) and Károly Róbert (Gyöngyös) and instead of faculties, it is divided into 21 institutes. 

Our team belongs to the Institute of Aquaculture and Environmental Safety, one of the largest institutes of the new university with approximately 150 employees and PhD students at 3 campuses. One of the constituent institutes of the former NAIK, the Research Institute for Fisheries and Aquaculture (HAKI) also belongs to our Institute now (renamed as a research center). The new Institute consists of 6 departments and one research center. Our team belongs to the Department of Aquaculture of which Ákos is the designated chair.

To know more about the new university, please visit this site: https://uni-mate.hu/hu 

Sampling in Lillafüred

 On January 27th 2021, we went to Lillafüred to sample the triploid rainbow trout that were transplanted with brown trout spermatogonia three years ago as well as tiger trout that were transplanted with rainbow trout spermatogonia as adults. The triploids have grown to an enormous size, some females were close to 5 kg in weight. Results of the transplantation will be verified by histology as well as using molecular markers. 

A triploid rainbow trout female transplanted with brown trout spermatogonia

Gyuri, the owner of the farm has also shown us the fish that were the result of the first successful transplantation of rainbow trout spermatogonia into tiger trout recipients. Sperm stripped from one of the tigers was used to fertilize rainbow trout eggs and as the photos show, the progeny are completely normal rainbow trout. Many of the males are already sexually mature and produced sperm that was used for regular fertilization.

A fish hatched from rainbow trout eggs fertilized with sperm stripped from a tiger trout recipient



12/07/2020

Our group's appearance in the Global Aquaculture Advocate


 An interview was published in the online journal Global Aquaculture Advocate with the participation of Professor Goro Yoshizaki of the Tokyo University of Marine Science and Technology and Zoran from our team. The article discusses the newest developments in the culture, cryopreservation and transplantation of germline stem cells (GSC) of fish. Enjoy!


9/18/2020

New paper published in Aquaculture Reports

 A new paper with Betti and Ákos as coauthors was published in the journal Aquaculture Reports. Enjoy!

Ljubobratović, U., Péter, G., Demény, F., Kugyela, N., Horváth, Á., Pataki, B., Horváth, Z., Jakabné Sándor, Z. & Rónyai, A. (2020). Reproductive performance in virgin pikeperch (Sander lucioperca L.) females fed different dietary levels of arachidonic acid with respect to the duration of spawning induction. Aquaculture Reports, 18, 100430.

Abstract

Pikeperch (Sander lucioperca) is a species of significant importance for the further development of intensive aquaculture, what makes the total control over species’ reproductive cycle a high priority task. The present study aimed to assess the effect of arachidonic acid (AA) dietary enrichment on the reproductive performance in virgin females (two-years-old; individual weight 1.0 ± 0.2 kg) kept in fully controlled conditions with respect to different time of hormonal application. Two groups of breeders, ARA (fed AA enriched diet) and CTRL (fed control feed), were reared in two separated tanks of the recirculation system over the course of seven months. Five, six, and seven months after the start of trial, batches of five pairs of breeders (in total 15 pairs per each feeding group) from each group were hormonally treated. Ovulations occurred after six and seven months (oocyte diameter range 912−1030 μm), and samples of dry eggs were taken for the analysis of fatty acid profiles. Among the parameters of reproductive success, the embryo survival was significantly affected by the diet, while induction duration affected latency time only. The CTRL group females showed overall higher embryo survival across both reproduction occasions (p = 0.028) than group fed AA enriched diet (35.7 ± 17.1 % and 18.6 ± 13.6 % in CTRL and ARA group, respectively). A significant accumulation of AA was found in ARA group eggs in both reproduction batches. Virgin pikeperch breeders appear to keep at least one month plasticity towards the duration of spawning induction in controlled conditions and optimal oocyte diameter range for artificial reproduction is between 900−1000 μm. Levelling dietary AA with eicosapentaenoic acid during the entire photothermal protocol negatively altered the egg quality.

New paper published in Dose-Response

 A new paper with Ákos' coauthorship was published in the journal Dose-Response. Enjoy!

Kerekes, F., Kollár, T., Gazsi, G., Kása, E., Urbányi, B., Csenki-Bakos, Z., & Horváth, Á. (2020). Investigation of Fertilizing Capacity of Zebrafish (Danio rerio) Sperm Exposed to Heavy Metals. Dose-Response. https://doi.org/10.1177/1559325820919597 

Abstract

The objective of our study was to investigate the effects of heavy metals on the fertilizing capacity of exposed zebrafish sperm, on embryonic survival, and on occurrence of embryonic deformities following fertilization with exposed sperm. It is important to test heavy metals because they are well-known pollutants. Sperm of externally fertilizing species can get in contact with pollutants found in aquatic environment. Zebrafish sperm, despite its advantages, has seldom been used in in vitro toxicological studies and no reports are available regarding the fertilizing capacity of exposed sperm. Zebrafish sperm was stripped and exposed to concentrations of the tested heavy metals (Zn2+, Cd2+, Cr3+, Cu2+, Ni2+, Hg2+, As3+) for 30 or 120 minutes. Calculated half-maximal effective concentration (EC50) values do not differ significantly from those calculated for motility for any of the tested heavy metals, which means fertilization rate can indicate the toxicity of the given substance following exposure of sperm. Thus, its application as in vitro toxicological end point is reasonable. The survival of embryos and embryonic development have not been affected by the exposure of spermatozoa, which means all alterations in spermatozoa caused by heavy metals have been expressed before 24 hours post fertilization.

New paper published in JoVE

 A new paper with Ákos' coauthorship was published in the Journal of Visualized Experiments (JoVE). Enjoy!

Csenki, Z., Horváth, Á., Bock, I., Garai, E., Kerekes, F., Vásárhelyi, E., Kovács, B., Urbányi, B., Mueller, F., Bakos, K. Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds. J. Vis. Exp. (162), e60462, doi:10.3791/60462 (2020).

Summary

Present here is a detailed protocol for the use of zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects. The protocol covers the propagation of the fish and treatment of embryos, and emphasizes the detection, documentation, and the evaluation of fluorescent signals induced by endocrine disrupting compounds (EDC).


7/30/2020

New paper published in Cryobiology

A new paper with Ilija's first authorship was published in the journal Cryobiology.

Šćekić, I., Marinović, Z., Lujić, J., Müller, T., Kitanović, N., Urbányi, B., Horváth, Á., 2020. A novel strategy for conservation of European eel (Anguilla anguilla) genetic resources: Cryopreservation of ovarian stem cells. Cryobiology 95, 151–156. https://doi.org/10.1016/j.cryobiol.2020.03.009

Enjoy!

Abstract:

The aim of this study was to develop short- and long-term preservation protocols for European eel ovarian stem cells (OSCs) through hypothermic storage and cryopreservation of ovarian fragments that will assist in current conservation programs of this critically endangered species. Firstly, a freezing procedure was developed by testing different cryomedia and technical aspects of freezing. Utilization of 1.5 M of dimethyl sulfoxide (Me2SO), 0.1 M glucose and 1.5% BSA yielded optimal OSCs survival. Additionally, equilibration of 50-mg ovarian fragments for 30 min and plunging into lN2 at −80 °C displayed the highest OSC viability. Different cooling rates ranging from −1 to −40 °C/min did not significantly affect OSC viability when thawing in a 10 °C water bath. In addition, application of needle-immersed vitrification (NIV), combining ES3 (1.5 M PG and 1.5 M Me2SO) with VS3 (3 M PG and 3 M Me2SO) yielded the highest viability rates. Finally, hypothermic storage (4 °C) of ovarian fragments and ovarian cell suspensions displayed favorable viability of ~90% after 48 h of storage and ~65% after 72 h of storage. The development of OSC preservation methods presents an onset of further development of germline stem cell (GSC) manipulation techniques in this species. Cryopreservation of OSCs can enable a continuous supply of cells for either transplantation or in vitro cell culture thus enabling new and improved management and conservation strategies for this endangered species.

5/12/2020

New book chapter published

A chapter was published with the authorship of Ákos and Dr. Béla Urbányi in the book entitled Reproduction in Aquatic Animals: From Basic Biology to Aquaculture Technology edited by Dr. Manabu Yoshida and Dr. Juan F. Asturiano and published by Springer. The book is the final result of the activities of the COST Action FA1205 AQUAGAMETE as well as the International Symposium on “AQUAGAMETE: Reproduction of Aquatic Animals” held in the Joint Meeting of the 22nd International Congress of Zoology and the 87th meeting of the Zoological Society of Japan, which was held from 14th to 19th November 2016 in Okinawa, Japan. The authors wish to thank Dr. Yoshida and Dr. Asturiano for taking care of the book and making it happen. Enjoy!

Horváth Á., Urbányi B. (2020) Chapter 15 Sperm Cryopreservation of Aquatic Species. In: Yoshida M., Asturiano J. (eds) Reproduction in Aquatic Animals. Springer, Singapore, https://doi.org/10.1007/978-981-15-2290-1_16

Cryopreservation of fish sperm is feasible, and methods have been developed for several hundred species. The procedure includes the collection of sperm and assessment of its quality, dilution in suitable extenders, addition of cryoprotectants, loading into freezing devices, cooling, storage, thawing, and finally post-thaw use of sperm. An alternative to traditional freezing methods is the vitrification of sperm which is promising primarily in smaller model fish species. A wide variety of protocols are available in the literature; however, in spite of the significant progress made by the scientific community, commercial application of fish sperm cryopreservation is still very limited.

New paper published in Fish and Shellfish Immunology

A new paper with Ákos' co-authorship was published in the journal Fish and Shellfish Immunology. The paper was a result of a collaboration among Dr. Daniel Żarski of the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, the team of Dr. Patrick Kestemont of the University of Namur in Belgium, Dr. Pascal Fontaine of the University of Lorraine in France and the Department of Aquaculture of Szent István University. It was also financed by the Hungarian-Walloon bilateral project TÉT_14_VL1-2015-0001. Enjoy!

Żarski, Daniel ; Ben Ammar, Imen ; Bernáth, Gergely ; Baekelandt, Sébastien ; Bokor, Zoltán ; Palińska-Żarska, Katarzyna ; Fontaine, Pascal ; Horváth, Ákos ; Kestemont, Patrick ; Mandiki, Syaghalirwa N.M. Repeated hormonal induction of spermiation affects the stress but not the immune response in pikeperch (Sander lucioperca). Fish and Shellfish Immunology 101 pp. 143-151. , 9 p. (2020)

Hormonal induction of spermiation, previously reported to be immunogenic in fishes, is a common hatchery practice in pikeperch, Sander lucioperca. The aim of the present study was to investigate the effects of repeated induction of spermiation in pikeperch, following application of either human chorionic gonadotropin (hCG) or salmon gonadoliberine analogue (sGnRHa) on sperm quality indices as well as on immune and stress response. Mature males of pikeperch (n = 7 per group) were stimulated twice with five days between injections of either hCG (hCG; 500 IU kg−1), sGnRHa (sGnRHa; 50 μg kg−1) or NaCl (control group; 1 ml kg−1) to assess spermatozoa motility with a computer-assisted sperm analysis (CASA) system. During second sampling, blood plasma was sampled for humoral innate immune (peroxidase and lysozyme activities, ACH50), stress (cortisol, glucose) and endocrine (testosterone) markers. In addition, the head kidney was dissected to assay the expression of several immune genes (such as il1, c3, hamp, tnf-α and lys genes). The results indicate that hormonal treatment significantly increased sperm production. Sperm sampled after the hormonal treatment maintained its quality throughout the study, regardless of the sampling time. However, it appears that the application of hCG induced elevated cortisol and glucose plasma levels compared to the control group. Almost all immune markers, except the relative expression of hepcidin (hamp gene), were unaffected by the two hormones applied. The results showed that the induction treatment of spermiation processes in pikeperch resulted in an important physiological stress response for which the intensity varied according to the hormonal agent used. However, this stress response (more profound following application of hCG) was weakly associated with innate immune functions. On the other hand, a significant negative correlation between the expression of several important immune markers (peroxidase activity, relative expression of c3 and il1 genes) and sperm quality indices indicates significant involvement of immune status on sperm quality. The results obtained shed light on immune-system- induced modifications to sperm quality. The data presented here highlight the need for careful revision of broodstock management and selection practices where welfare status as well as individual predispositions of fish to cope with the stress should be taken under the consideration.

5/11/2020

Conference in Croatia

The 55th Croatian & 15th International Symposium on Agriculture was held on February 16-21st, 2020, in Vodice, Croatia. Ákos has given a presentation entitled:
Ákos Horváth, Jelena Lujić, Zoran Marinović, Márton Hoitsy, Béla Urbányi, György Hoitsy:
The use of the hybrid tiger trout as a recipient for interspecific transplantation of spermatogonia

Ákos giving the presentation (photo by R. Balogh)

A trip to Poland

Although the COVID-19 pandemic has anchored us to Hungary without much opportunities to travel, on February 7-9th, thus before the outbreak we (Nevena, Zoran and Ákos) managed to visit our friend, Dr. Daniel Żarski of the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences. We also had the opportunity to meet Dr. Andrzej Ciereszko, head of Gamete and Embryo Biology group of the institute and members of his team. Our mission was to bring around 100 Atlantic salmon juveniles to Hungary for scientific experiments on their gonads. The fish arrived successfully after a 14-hour journey.

We wish to thank Daniel, Andrzej, Sylwia and all our friends in Poland for their help!

Strengthening the Polish-Hungarian-Serbian friendship at the dinner (photos by D. Żarski)


1/15/2020

Collaboration with Cornell University

In June-August, 2019 we had the pleasure of hosting Logan Goddard, undergraduate student of the College of Agriculture and Life Sciences (CALS) of Cornell University on a 8-week stay in our lab. Logan has participated in the CALS Global Fellows Program and took part in experiments on cryopreservation of eel gonads and carp sperm. He gave an account of his experiences at the 2019 Global Fellows Program Showcase in November 2019. You can read more about this program here: https://cals.cornell.edu/news/global-fellows-program-celebrates-first-year-undergraduate-research-experiences-hungary

10/20/2019

Defense of Patrícia Diogo

On 18th October, Ákos has participated as a member of the examination committee in the defense of the dissertation entitled “Cryopreservation of zebrafish germ cells: technological improvements and methodological standardization for gene banking and management” by Patrícia Diogo at the University of Algarve in Faro, Portugal. Patrícia was a PhD student of our friends Dr. Elsa Cabrita and Dr. Paulo Gavaia and a member of the Aquagroup at the same university and the Centre of Marine Sciences (CCMAR). She gave an excellend presentation of her results and convinced the committee of her profound knowledge in the area of her studies. Congratulations, Dr. Diogo!

Patrícia Diogo (center) with members of the committee (Dr. Paulo Gavaia, Ákos, Dr. Elsa Cabrita, Dr. Catarina Oliveira, Dr. Juan F. Asturiano and Dr. Sérgio Jesus) following the defense

10/03/2019

New paper published in Scientifc Reports

A new paper with the first authorship of Zoran was published in Scientific Reports. Enjoy!

Zoran Marinović, Qian Li, Jelena Lujić, Yoshiko Iwasaki, Zsolt Csenki, Béla Urbányi, Goro Yoshizaki & Ákos Horváth: Preservation of zebrafish genetic resources through testis cryopreservation and spermatogonia transplantation. Scientific Reports volume 9, Article number: 13861 (2019)

Abstract

Zebrafish is one of the most commonly used model organisms in biomedical, developmental and genetic research. The production of several thousands of transgenic lines is leading to difficulties in maintaining valuable genetic resources as cryopreservation protocols for eggs and embryos are not yet developed. In this study, we utilized testis cryopreservation (through both slow-rate freezing and vitrification) and spermatogonia transplantation as effective methods for long-term storage and line reconstitution in zebrafish. During freezing, utilization of 1.3 M of dimethyl sulfoxide (Me2SO) displayed the highest spermatogonia viability (~60%), while sugar and protein supplementation had no effects. Needle-immersed vitrification also yielded high spermatogonia viability rates (~50%). Both optimal slow-rate freezing and vitrification protocols proved to be reproducible in six tested zebrafish lines after displaying viability rates of >50% in all lines. Both fresh and cryopreserved spermatogonia retained their ability to colonize the recipient gonads after intraperitoneal transplantation of vasa::egfp and actb:yfp spermatogonia into wild-type AB recipient larvae. Colonization rate was significantly higher in dnd-morpholino sterilized recipients than in non-sterilized recipients. Lastly, wild-type recipients produced donor-derived sperm and donor-derived offspring through natural spawning. The method demonstrated in this study can be used for long-term storage of valuable zebrafish genetic resources and for reconstitution of whole zebrafish lines which will greatly improve the current preservation practices.

Ákos promoted into professorship

By the Decree 351/2019. (VIII. 30.) of the President of the Republic of Hungary Ákos was appointed as a professor as of 1st September, 2019.

7th International Workshop on the Biology of Fish Gametes

Ákos, Timi, Betti and Nevena have participated at the 7th International Workshop on the Biology of Fish Gametes that was organized between 2-6th September in Rennes, France. As always, the workshop was a wonderful opportunity for scientist working on fish gametes to meet in person and share their newest results. Ákos, Timi and Nevena gave oral presentations durind the sessions while Betti presented a poster. We would like to thank the French colleagues for the wonderful experience and professional organization. Looking forward to seeing everyone in two years in Poland.

Oral presentations by the group:

Tímea Kollár, Bernadett Pataki, Gyöngyi Gazsi, Béla Urbányi, Ákos Horváth: Effect of age on the sensitivity of zebrafish (Danio rerio) sperm
Ákos Horváth, Zoran Marinović, György Hoitsy, Márton Hoitsy, Boglárka Hoitsy, Béla Urbányi, Jelena Lujić: Is the hybrid tiger trout a good recipient for the transplantation of early-stage germ cells?
Ilija Šćekić, Nevena Kitanović, Zoran Marinović, Tamás Müller, Béla Urbányi, Jelena Lujić, Ákos Horváth: Preservation of ovarian tissue in european eel (Anguilla anguilla)

Posters presented or co-authored by the group:

Roman Franěk, Taiju Saito, Tomáš Tichopád, Michaela Fučíková, Zoran Marinović, Jelena Lujić, Ákos Horváth, Vojtěch Kašpar, Martin Pšenička: Germ cell manipulation as a tool for common carp isogenic lines production and management.
Bernadett Pataki, Tímea Kollár, Zoran Marinović, Jelena Lujić, Gyöngyi Gazsi, Roberta Izabella Berta, Béla Urbányi, Ákos Horváth: Inheritance of sperm cryoresistance in zebrafish (Danio rerio)


7/06/2019

Frontires in Reproduction: Molecular and Cellular Concepts and Applications (FIR)

Jelena spent six weeks at the Marine Biological Laboratory (University of Chicago) participating in the course Frontires in Reproduction: Molecular and Cellular Concepts and Applications (FIR). During that time, she had lectures in all relevant methods in reproductive biology such as endocrinology, stem cells, gametogenesis, fertilization, sex determination etc. Most of the time was devoted to laboratory practice and performing experiments regarding signalling, CRISPR Cas9, epigenetics, in situ hybridization, tissue and oocyte culture, germ cell transplantation, oocyte maturation, ICSI, IVF, embryo transfer, ESC microinjection, reproductive immunology, placentation etc.
Beside the educational part, this was the perfect opportunity for networking. The course itself has a very long tradition (since 1998) and the FIR community is very significant in the area of reproductive biology and includes scientists from around the world (circa 400 people).
Beside the support of our University, Jelena`s participation was partly funded by the NICHD and the Burroughs Wellcome Fund.
Jelena can now proudly say that she is a FIRbee 😊

FIRbees 2019
FIRbees 2019 with section directors and organizers
Working mode
Mission accomplished - sperm lab
Science is exciting



Chalk talk about fish surrogate production
Wrap up of the labs from the Section 1
Jelena`s presentation at FIR symposium 2019
FIRbees 2019 at the sunset in the Woods Hole
FIRbees 2019 at the closing reception
FIRbees 2019 at the closing reception
FIRbees during the weekend
It is written in stone, no, in the tissue
Grill party and fun
We are big happy family now
Present from dear colleague Augustin