CEEPUS Summer Course 2016 - New fields, advances and innovation in aquaculture and fisheries

Our deparment has organized a CEEPUS Summer School between 04/July/2016 and 09/July/2016.

The members of our group also participated with giving lectures and practices (listed below) to the students of the summer school.

Seminars:

• Dr. Urbányi, Béla: Introduction of world and European aquaculture production, outlines of problems and breakout opportunities, trends and development directions, general description of biotechnology and genetic processes used in fish farming.
• Dr. Horváth, Ákos: Cryopreservation in aquatic species.
• Dr. Jelena Lujic: Germ cell transplantation as a novel technique in aquaculture and fish conversation
• Zoran Marinovic: Isolation and transplantation of fish primordial germ cells and spermatogonia

Parctices:

• Fish sperm cryopreservation in practice (Bernáth, Gergely, Kása, Eszter)
• Methodology and work on molecular biology techniques (Dr. Kovács, Balázs, Ősz,Ágnes and Guti, Csaba)
• Spermatogonia isolation (Zoran Marinovic)

Some pictures from the course:

Presentation of Ákos

Practical class given by Gergő and Eszti

Gergő during cryopreservation practice

Jelena's presentation

Jelena and the students

practice with Zoran and Jelena

Zoran's presentation

happy group :)

Another paper accepted in General and Comparative Endocrinology

A new paper by our group with the first authorship of Zoran Marinović was accepted for publication in General and Comparative Endocrinology as a part of the proceedings of last year's 5th International Workshop on the Biology of Fish Gametes held in Ancona, Italy on September 7-11, 2015. For more information please consult this link: http://www.sciencedirect.com/science/article/pii/S0016648016302027

Cryosurvival of isolated testicular cells and testicular tissue of tench Tinca tinca and goldfish Carassius auratus following slow-rate freezing

Abstract

Experiments were carried out to test the efficiency of cryopreservation of whole testicular tissue in tench Tinca tinca and goldfish Carassius auratus and compare it to cryopreservation of isolated testicular cells. Additionally, effects of three cryoprotectants (dimethyl sulphoxyde – Me2SO, methanol – MeOH and ethylene glycol – EG) at three concentrations (1 M, 2 M and 3 M) on post-thaw cell viability were assessed. Tissue pieces / isolated testicular cells were diluted in cryomedia and cryopreserved by slow-rate freezing (1 °C/min to – 80 °C followed by a plunge into the liquid nitrogen). In both species Me2SO and EG generally yielded higher cryosurvival of early-stage germ cells than MeOH, while spermatozoa of neither species displayed such a pattern. In most cases a 3M > 2M > 1M viability pattern emerged in both species for both sample types regardless of the cryoprotectant used. Sample type (dissociated testicular cells vs testicular tissue) did not seem to affect viability rates of tench early-stage germ cells and goldfish spermatozoa, while the opposite was observed for tench spermatozoa and goldfish early-stage germ cells. Additionally, through histological analysis we displayed that tissue structure mainly remained unaltered after thawing in goldfish. These results indicate that cryopreservation of whole testicular tissue is indeed a valid alternative method to cryopreservation of dissociated testicular cells. Early-stage germ cells obtained from cryopreserved testis can be further used in different purposes such as transplantation into suitable donors while viable sperm might be used for fertilization when feasible.