Fish Reproduction Group: March 2018

3/22/2018

HU-SRB Bilateral project "Invasive and blooming cyanobacteria in Serbian and Hungarian waters - a threat to fish?"

From March 14^th until 17^th, Eszti, Jelena and Ilija Šćekić (Master student in our group) were in Serbia as a part of our HU-SRB Bilateral project "Invasive and blooming cyanobacteria in Serbian and Hungarian waters - a threat to fish?".

In this project, we collaborate with our dear colleagues from the University of Novi Sad lead by prof. dr Zorica Svirčev. During their stay, they were hosted by colleagues from the Laboratory for Paleoenvironmental Research (LAPER) and Laboratory for Hydrobiology, Department of Biology and Ecology, Faculty of Sciences, Novi Sad.

Our team had opportunity to visit Novi Sad Cyanobacterial Culture Collection (NSCCC) and Chamber for experimental research of BLOCDUST hypothesis that are part of LAPER long-term projects at the Department in Novi Sad.

The main goal of the visit was fieldwork and the first sampling of water, phytoplankton and fish on the Special Nature Reserve "Ludaš" for this year. Various fish tissues were collected for further histological analysis and examination of possible effects of continued water blooming that occurs in that lake.

After the successful field work, we got invitation to visit Palić Zoo where we were guests of Kristian Ovari, the main biologist at the Zoo. We spent nice time there in a friendly atmosphere; thank you Kristian.

We would also like to thank our hosts, especially dr Nada Tokodi, dr Damjana Drobac, Sándor Sipos and Nemanja Pankov for perfect organization, joint work and valuable support and help on the field.

Nemanja measuring physical-chemical parameters of the water

Nemanja taking water samples

Nemanja collecting phytoplankton

It is time for fish sampling

Sándor and Nemanja are ready...

...and go

Tissue collecting

Samples are ready

Chamber for experimental research of BLOCDUST hypothesis

Visit to the Department of Biology and Ecology with dr Desanka Kostić, dr Damjana Drobac and dr Nada Tokodi

Novi Sad Cyanobacterial Culture Collection (NSCCC)

Visit to Palić Zoo with our dear host Krstijan Ovari

Palić Zoo

3/21/2018

New paper accepted in Fish Physiology and Biochemistry

A new paper in collaboration with our collegues from the Polish Academy of Sciences was accepted for publication in Fish Physiology and Biochemistry, entitled 'Optimisation of sodium and potassium concentrations and pH in the artificial seminal plasma of common carp Cyprinus carpio L.'.

Beata Irena Cejko, Ákos Horváth, Tímea Kollár, Eszter Kása, Jelena Lujić, Zoran Marinović, Béla Urbányi, Radosław Kajetan Kowalski
DOI: https://link.springer.com/article/10.1007%2Fs10695-018-0491-3

Abstract
The effect of sodium and potassium concentrations as well as optimal pH on the motility of common carp Cyprinus carpio L. sperm during shortterm storage in artificial seminal plasma (ASP) was investigated. Sperm was collected from individual males (n = 5) and each sample diluted tenfold (1:9) in ASP (sperm:extender) containing 2 mM CaCl2, 1 mM Mg2SO4 and 20 mM Tris at pH 8.0 and supplemented by the following concentrations of sodium and potassium (mM/mM): 0/150, 20/130, 40/ 110, 75/75, 110/40, 130/20 and 150/0. The osmolality of all ASP variants was set at 310 mOsm kg−1 . Sperm motility was measured using a CASA system during 72 h of storage. Immediately after dilution, sperm motility was high (90%) both in each variant and in the control group (fresh sperm). After 72-h storage, the highest sperm motility was noted in ASP containing 110 mM NaCl and 40 mM KCl. No differences were found in the motility of samples preserved within the pH range of 7.0–9.0. Our data suggest that for the short-term storage of common carp sperm, whereas the pH of the solution does not play a crucial role, a specific potassium concentration of around 40 mM is required.

3/20/2018

23rd Biotechnology Conference in Serbia

On 9^th and 10^th March our team participated at the 23^rd Biotechnology Conference organized by Agronomy Faculty, University of Kragujevac, Serbia. We used the opportunity to present the results of our current project.

Lujić J., Marinović Z., Kása E., Šćekić I., Urbányi B., Horváth Á. "Surrogate production in freshwater fish", 23^rd Biotechnology Conference, 9-10.March 2018; Čačak, Serbia.pp.123.