A new paper of our group was accepted for publication in Fish Physiology and Biochemistry, entitled "In vitro toxicology test system based on common carp (Cyprinus carpio) sperm analysis".
Abstract
The effect of heavy metals on the motility parameters of common carp
sperm was investigated. In vitro test systems are widespread in
ecotoxicology, and fish sperm can be a suitable model. For this reason,
studies had been carried out in this topic; however, the published
methods are not standard in several aspects (donor species, measured
endpoint, etc.). In this study, a previously published toxicology-aimed
sperm analysis protocol was tested to examine the effect of heavy metals
(arsenic, cadmium, chromium, copper, mercury, nickel, zinc,) on common
carp sperm. According to our results, PMOT is the most sensitive of the
investigated parameters: dose-response was observed in case of each
metal at low concentrations, already after 30 min of exposure. VCL was
less sensitive: lower effects were observed at the same concentrations
compared to PMOT. Among the examined parameters, LIN was the least
affected: a dose-response was observed only in case of arsenic and
mercury. The same sensitivity of motility parameters was observed on
zebrafish sperm previously. Moreover, we found that PMOT, VCL, and LIN
of common carp sperm were affected at the same concentrations as it had
been observed in zebrafish, when the identical analytical protocol was
applied. The only exception was As3+, where common carp sperm
proved to be more sensitive: lower concentrations already reduced its
motility parameters. Consequently, PMOT of common carp sperm is an
accurate and fast bioindicator of aquatic pollution.
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