54th Croatian and 14th International Symposium on Agriculture

All members of our group have participated at the 54^th Croatian and 14^th International Symposium on Agriculture, organized in Vodice, Croatia, in the period between 17^th and 22^nd February. During the conference, we presented seven papers as an oral presentations and one poster.

We  also had opportunity to visit Marine research center of the Institute Ruđer Bošković,Zagreb.

Thank organizers for the invitation, we are looking forward to meet again.

1. Tímea Kollár, Balázs Csorbai, Béla Urbányi, Zsolt Csenki-Bakos, Ákos Horváth. Fish sperm as in vitro toxicology test system Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia. pp. 142.

2. Horváth Á., Marinović Z., Šćekić I., Simona Sušnik Bajec, Ida Djurdjevič, György Hoitsy, Urbányi B., Lujić J.. 2019. Transplantation of freshwater fish spermatogonia. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia.pp. 160.

3.  Horváth Á. GOODFISH – A project for the development of Hungarian aquaculture. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia.pp. 159.

4. Balázs Kovács, Réka Balogh, Szilvia Keszte, Lujić J., Gábor Szilágyi, Milán Varjú, Bence Schmidt-Kovács, Balázs Csorbai, Urbányi B. 2019. Development of an African catfish (Clarias gariepinus) breeding system with molecular genetic support. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia. pp. 163.

5.  Lujić J., Marinović Z., Šćekić I., Urbányi B., Horváth Á. 2019. Conservation of female genetic material in fish. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia. pp. 164.

6.  Marinović Z., Lujić J., Franěk R., Urbányi B., Fučíková M., Kašpar V., Pšenička M., Horváth Á. 2019. Cryopreservation and transplantation of common carp germ cells. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia. pp. 165.

7.  Pataki B., Kollár T., Z Marinović Z., Lujić J., Gazsi G., Berta R. I., Urbányi B., Horváth Á.. 2019. Zebrafish (Danio rerio) sperm cryopreservation. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia. pp. 170.

8.  Šćekić I., Lujić J., Marinović Z., Urbányi B., Horváth Á. 2019. Cryopreservation of eel gonadal tissue. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia. pp. 174.

Ákos during his presentation

Jelena during her presentation

Zoran during his presentation

Ilija during his presentation

Betti during her presentation

Timi presenting her poster

Visit to Marine research center of the Institute Ruđer Bošković

Visit to Marine research center of the Institute Ruđer Bošković

New paper accepted in Cyobiology

A new paper in collaboration with our colleagues from University of South Bohemia in Česke Budejovice, Faculty of Fisheries and Protection of Waters (Czech Republic) has been accepted for publication in Cryobiology.

 

Roman Franěk, Tomáš Tichopád, Christoph Steinbach, Xuan Xie, Jelena Lujić, Zoran Marinović, Ákos Horváth, Vojtěch Kašpar, Martin Pšenička. Preservation of female genetic resources of common carp through oogonial stem cell manipulation. Cryobiology. DOI:10.1016/j.cryobiol.2019.01.016

Abstract

Several experiments were conducted in order to develop an optimal protocol for slow-rate freezing (-1°C/min) and short-term storage (-80 or 4 °C) of common carp oogonial stem cells (OSCs). Dimethyl sulfoxide with concentration of 1.5 M was identified as the best cryoprotectant in comparison to propylene glycol and methanol. When comparing supplementation of sugars (glucose, trehalose, sucrose) in different concentrations (0.1, 0.3, 0.5M), glucose and trehalose in 0.3 M were identified as optimal. Finally, different equilibration times revealed a positive impact of prolonged equilibration on post-thaw viability. Short-term storage options for ovarian tissue pieces at -80 °C and 4 °C were tested as alternatives to cryopreservation and storage in liquid nitrogen. The presence of oogonia was confirmed by immunocytochemistry and viability after storage was determined by the trypan blue exclusion test. This study identified the best protocol for OSC cryopreservation using slow rate freezing resulting in ~65% viability. The frozen/thawed OSCs were labelled by PKH-26 and transplanted into goldfish recipients. The success of the transplantation was confirmed by presence of fluorescent cells in the recipient gonad and later on by RT-PCR with carp vasa specific primers.

New paper accepted in Zebrafish

A new paper with the shared first authorship of Timi and with the shared correspondence authorship of Ákos has been accepted for publication in Zebrafish. This work has been achieved with our colleagues from the University of Sao Paolo. 

Caetano Da Silva, C., Kollár, T., Csenki-Bakos, Zs., Fernandes, B.H.V., Horváth, Á., Carvalho, L.R. (2019): An easy method for cryopreservation of zebrafish (Danio rerio) sperm. Zebrafish, doi: 10.1089/zeb.2018.1696

Abstract
We developed an easy, efficient, and cheap protocol for zebrafish sperm cryopreservation carried out on dry ice (20 min) using simple composition solution (200mM glucose, 40mM KCl, 30mM Tris, pH = 8.0). The average efficiency of the present cryopreserve method was between 10% and 20% (expressed as fertilization rate). The experiments were conducted and repeated at two different locations, in different countries, yielding very similar results, showing the reproducibility and applicability of the method.

Keywords: zebrafish, Danio rerio, sperm, cryopreservation, dry ice