A new paper with the shared first authorship of Timi and with the shared correspondence authorship of Ákos has been accepted for publication in Zebrafish. This work has been achieved with our colleagues from the University of Sao Paolo.
Caetano
Da Silva, C., Kollár, T., Csenki-Bakos, Zs., Fernandes, B.H.V., Horváth,
Á., Carvalho, L.R. (2019): An
easy method for cryopreservation of zebrafish (Danio
rerio)
sperm. Zebrafish, doi: 10.1089/zeb.2018.1696
Abstract
We developed an easy, efficient, and cheap protocol for zebrafish sperm cryopreservation carried out on dry ice (20 min) using simple composition solution (200mM glucose, 40mM KCl, 30mM Tris, pH = 8.0). The average efficiency of the present cryopreserve method was between 10% and 20% (expressed as fertilization rate). The experiments were conducted and repeated at two different locations, in different countries, yielding very similar results, showing the reproducibility and applicability of the method.
Keywords: zebrafish, Danio rerio, sperm, cryopreservation, dry ice
Fish Reproduction Group (Hungarian University of Agriculture and Life Sciences, Institute of Aquaculture and Environmental Safety, Department of Aquaculture)
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