Also, we carried out isolation and cryopreservation of marble trout (Salmo marmoratus) and grayling testes and ovaries. The objective of these studies is to use these gonads later for isolation of spermatogonia and oogonia. These can then be transplanted into suitable recipients, in this case triploid rainbow trout larvae. At the moment we are waiting for a shipment of mixed-sex triploid rainbow trout embryos, thus, the gonads will have to stay frozen until these arrive.
Finally, after a one-month stay, we had to say goodbye to our two students from the University of Novi Sad, Nataša Cvetković and Jovana Lovren. They endured an incredibly tough one month of intensive training in cryopreservation, transplantation, histology and immuno-histochemistry in our lab and did a very good job. Thank you, Nataša and Jovana, and hope to see you soon in Hungary for new adventures!
We would also like to express our gratitude to Mr. Dušan Jesenšek, manager of aquaculture activities of the Angling Club of Tolmin for his help and guidance, as well as to Dr. Simona Sušnik-Bajec, Dr. Aleš Snoj and Ida Djurdjevič of the University of Ljubljana for their participation in the experiments.
|Dissection of grayling juveniles for the isolation of gonads|
The first grayling male
Cryopreservation on the field