Autumn field work in Slovenia

Ákos, Gergő and Eszti were in Tolmin, Slovenia for sperm cryopreservation and vitrification work on the Marble trout (Salmo marmoratus) from 16-11-2015 to 20-11-2015.

A part of the experimental fish were supplied by the Angling Association of Tolmin, the other part was collected from the natural habitat (a genetically pure population) of this species.

marble trout selection at the fish farm of the Angling Association

marble trout

fish waiting for sampling

during anesthesia (top: marble trout, bottom: hybrid trout from the Rižana river)

sperm collection for cryopreservation experiments

egg collection for fertilisation tests

our team at work

on the way to Huda Grapa, the natural habitat of a pure Marble trout population

searching for fish below a waterfall

Gergő climbing up

Dušan climbing up

still collecting fish (from left: Elvis, Dušan, Ákos)

We caught 8 male marbles, let's start stripping the sperm!

anasthesia in the field

sperm stripping

these fish are tagged with ID numbers, all of these individuals are investigated for their life history and genetics by our collegues from the Biological Station Tour de Valat in France and the University of Ljubljana

Gergő was releasing the fish after stripping

Dušan and Ákos during sperm collection

samples stored in a box, waiting for cryopreservation

cryopreservation in the field (Gergő and Ákos pouring liquid nitrogen onto frozen samples)

We had the opportunity to visit the new trout farm in Tolmin, which has just opened in the summer of 2015.

tank full of trouts

the main building has "scales" and the fish tanks have the shape of a fish body

the farm is equipped with a modern processing system, here Dusan is showing the smoking chamber

And last but not least, a video about stripping the sperm of Marble Trout at Huda Grapa:

Transplantation work in Slovenia part 1

Jelena and Zoran were in Slovenia at the Department for Animal Science, Biotechnical Faculty of Ljubljana. With our colleagues Simona, Ida, Aleš and Nejc they did preliminary gonadal cryopreservation experiments and preparation of the tissue for transplantation on trout species. 

Hungarian/Slovenian team (from the left: Ida, Aleš, Simona, Jelena and Zoran)

at the trout farm

it is always good time to get some new, interesting information

work at the trout farm

packing the fish - it is time for the lab

work in the lab

work in the lab

atmosphere in the lab with dr Saša Marić (University of Belgrade, Serbia) and Mateja Janeš (University of Zagreb, Croatia)

happy face by Zoran

happy face by Nejc

storyteller on the Lake Bled

 

Introduction of our student Isabela Di Chiacchio

Isabela is spending one semester at the Szent István University (from September 2015 until February 2016).

She started her MSc studies in 2014 in Brazil (Federal University of Lavras, Department of Animal Sciences), her research subject is the cryopreservation of fish sperm, and she has experiences in brazilian native species reproduction. She has a BSc degree in Veterinary Medicine (Federal University of Lavras - Brasil, 2013). 

Isabela is participating in the experiments of the Fish Reproduction Group, she is helping our work on the fields and also in the lab. She takes part mainly in the vitrification experiments of Eszti.

Gonad- and skin sample collection at Lillafüred Trout Farm

On the 13th of October we went for a field trip to Lillafüred in the company of Slovenian and Brazilian collegues. For diverse ongoing experiments of the researchers from the group, different samples were taken from the tiger trout individuals. Tiger trout is the hybrid of the species brown- and brook trout species and has a special pattern on the skin.

tiger trout individual

fish farm with golden trouts

tiger trout for sampling

Gyuri, the leader of the trout farm is catching some fish for us

the first fish is ready for sampling (from left: Isabela, our Brazilian MSc student, Ida and Simona; Slovenian collegues from the University of Ljubljana, and Jelena with Zoran)

checking the sample

celebrity :)

Eszti and Isabela with golden trouts :)

European Congress of Ichthyology XV

We have participated at the 15th European Congress of Ichthyology, organized by the Interdisciplinary Centre of Marine and Environmental Research (CIIMAR) in Porto, Portugal, from 07-09-2015 to 11-09-2015. 

From our group Ágnes has participated in the conference with oral presentation and from the our department Árpad Hegyi has presented his results as a poster.

Ágnes

 

 Árpád

The results that our group has presented at the conference:

Oral presentation:

Ősz Á, Kovács B, Kánainé Sipos D, Hoitsy Gy, Snoj A, Keszte Sz, Sáfrány AJ, Herzinyák Z, Kaczkó D, Palkó Cs, Urbányi B, Horváth Á (2015): Genetic background and population genetics of Hungarian brown trout populations using PCR-RFLP and microsatellite markers. (oral presentation) 15th European Congress of Ichthyology, Porto, Portugal, 7-11. September 2015, Book of abstracts p. 127-128.

5th International Workshop on the Biology of Fish Gametes

We have participated at the 5th International Workshop on the Biology of Fish Gametes, organized by the Faculty of Agriculture at the University of Marche in Ancona, Italy, from 07-09-2015 to 11-09-2015. 

The results that we have presented at the conference:

Oral presentations:

●Bernáth G, Kása E, Szentes K, Staszny A, Várkonyi L, Kollár T, Hegyi A, Bokor Z, Urbányi B, Horváth A. IMPROVEMENT OF COMMON CARP (Cyprinus carpio) SPERM CRYOPRESERVATION: THE APPLICABILITY OF A CONTROLLED-RATE FREEZER AS A STANDARD DEVICE.

●Horváth A, Bernáth G, Bokor Z, Kása E, Ősz A, Urbányi B, Cabrita E, Gavaia P, Snoj A, Bravničar N, Jesenšek D. PROGRESS TOWARDS STANDARDIZATION OF CRYOPRESERVATION METHODS FOR SALMONID SPECIES OF THE ADRIATIC DRAINAGE BASIN.

●Kása E, Bernáth G, Kollár T, Żarski D, Lujić J, Marinović Z, Bokor Z, Hegyi A, Urbányi B, Vílchez MC, Morini M, Peñaranda DS, Pérez L, Asturiano JF, Horváth A. VITRIFICATION OF FISH SPERM. 

●Marinovic Z, Lujic J, Bernáth G, Urbányi B, Horváth Á. CRYOPRESERVATION OF TENCH Tinca tinca L. ISOLATED TESTICULAR CELLS. 

Poster presentations:

●Lujic J, Bernáth G, Marinovic Z, Radojkovic N, Simic V, Cirkovic M, Urbányi B, Horváth Á. EFFECTS OF CRYOPROTECTANTS IN DIFFERENT CONCENTRATIONS ON SPERM MOTILITY AND FERTILIZNG CAPACITY OF TENCH Tinca tinca SPERM.

●Kollár T, Horváth Á, Labbese C, Milon P, Csenki Z, Kovács R, Bernáth G, Béres T, Depincé A, Urbányi B, Labbé C. DEVELOPMENT OF CRYOPRESERVATION OF ZEBRAFISH (Danio rerio) SPERM.

conference registration 

Ákos as chairmen, Dr. Asturiano is presenting

Simona and Ales (our slovenian collegues from the University of Ljubljana) with Ákos

Zoran 

Gergő 

Ákos 

Eszti 

Timi 

Zoran

group picture (from left: Zoran, Jelena, Timi, Ákos, Eszti, Gergő) 

our group with hungarian flag