Video protocol for zebrafish testis vitrification

During the previous period, we have prepared a video protocol for the video journal JoVE https://www.jove.com/ regarding vitrification of zebrafish testis. This publication is supported by the COST Action FA 1205 AQUAGAMETE https://aquagamete.webs.upv.es/ It was a really nice and new experience for the whole team. 

Short term scientific mission (STSM) in Valencia

Timi and Eszti spent 10 days at the Polytechnic University of Valencia, working with the research team of Dr. Juan Asturiano. Our stay was financed by the AQUAGAMETE COST action.

The main topic of our investigations was the comparison of protocols for European eel (Anguilla anguilla) sperm cryopreservation, furthermore new developments in this topic were also carried out. The results of these experiments are going to be published in the JoVE, for this purpose all the methods were recorded on video.

Timi injecting an eel with hormon (to induce spermiation)

Eszti and Germán during stripping

sperm stripping

recording the steps of cryopreservation

Victor evaluating the sperm motility with CASA

cryopreservation

weekend lab meeting at the central market of Valencia
(from left to right: Timi, Luz, Johnny, Laura :), Chris, Filipa)