54th Croatian and 14th International Symposium on Agriculture

All members of our group have participated at the 54^th Croatian and 14^th International Symposium on Agriculture, organized in Vodice, Croatia, in the period between 17^th and 22^nd February. During the conference, we presented seven papers as an oral presentations and one poster.

We  also had opportunity to visit Marine research center of the Institute Ruđer Bošković,Zagreb.

Thank organizers for the invitation, we are looking forward to meet again.

1. Tímea Kollár, Balázs Csorbai, Béla Urbányi, Zsolt Csenki-Bakos, Ákos Horváth. Fish sperm as in vitro toxicology test system Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia. pp. 142.

2. Horváth Á., Marinović Z., Šćekić I., Simona Sušnik Bajec, Ida Djurdjevič, György Hoitsy, Urbányi B., Lujić J.. 2019. Transplantation of freshwater fish spermatogonia. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia.pp. 160.

3.  Horváth Á. GOODFISH – A project for the development of Hungarian aquaculture. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia.pp. 159.

4. Balázs Kovács, Réka Balogh, Szilvia Keszte, Lujić J., Gábor Szilágyi, Milán Varjú, Bence Schmidt-Kovács, Balázs Csorbai, Urbányi B. 2019. Development of an African catfish (Clarias gariepinus) breeding system with molecular genetic support. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia. pp. 163.

5.  Lujić J., Marinović Z., Šćekić I., Urbányi B., Horváth Á. 2019. Conservation of female genetic material in fish. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia. pp. 164.

6.  Marinović Z., Lujić J., Franěk R., Urbányi B., Fučíková M., Kašpar V., Pšenička M., Horváth Á. 2019. Cryopreservation and transplantation of common carp germ cells. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia. pp. 165.

7.  Pataki B., Kollár T., Z Marinović Z., Lujić J., Gazsi G., Berta R. I., Urbányi B., Horváth Á.. 2019. Zebrafish (Danio rerio) sperm cryopreservation. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia. pp. 170.

8.  Šćekić I., Lujić J., Marinović Z., Urbányi B., Horváth Á. 2019. Cryopreservation of eel gonadal tissue. Abstract book. 54th Croatian and 14th International Symposium on Agriculture; 17-22 February 2019, Vodice, Croatia. pp. 174.

Ákos during his presentation

Jelena during her presentation

Zoran during his presentation

Ilija during his presentation

Betti during her presentation

Timi presenting her poster

Visit to Marine research center of the Institute Ruđer Bošković

Visit to Marine research center of the Institute Ruđer Bošković

New paper accepted in Cyobiology

A new paper in collaboration with our colleagues from University of South Bohemia in Česke Budejovice, Faculty of Fisheries and Protection of Waters (Czech Republic) has been accepted for publication in Cryobiology.

 

Roman Franěk, Tomáš Tichopád, Christoph Steinbach, Xuan Xie, Jelena Lujić, Zoran Marinović, Ákos Horváth, Vojtěch Kašpar, Martin Pšenička. Preservation of female genetic resources of common carp through oogonial stem cell manipulation. Cryobiology. DOI:10.1016/j.cryobiol.2019.01.016

Abstract

Several experiments were conducted in order to develop an optimal protocol for slow-rate freezing (-1°C/min) and short-term storage (-80 or 4 °C) of common carp oogonial stem cells (OSCs). Dimethyl sulfoxide with concentration of 1.5 M was identified as the best cryoprotectant in comparison to propylene glycol and methanol. When comparing supplementation of sugars (glucose, trehalose, sucrose) in different concentrations (0.1, 0.3, 0.5M), glucose and trehalose in 0.3 M were identified as optimal. Finally, different equilibration times revealed a positive impact of prolonged equilibration on post-thaw viability. Short-term storage options for ovarian tissue pieces at -80 °C and 4 °C were tested as alternatives to cryopreservation and storage in liquid nitrogen. The presence of oogonia was confirmed by immunocytochemistry and viability after storage was determined by the trypan blue exclusion test. This study identified the best protocol for OSC cryopreservation using slow rate freezing resulting in ~65% viability. The frozen/thawed OSCs were labelled by PKH-26 and transplanted into goldfish recipients. The success of the transplantation was confirmed by presence of fluorescent cells in the recipient gonad and later on by RT-PCR with carp vasa specific primers.

Project meeting in Valencia

In parallel with Jelena and Ilija’s lab work in Valencia Ákos and our colleague Tamás Müller have participated at the second preparatory meeting of the MSCA ITN project EEL-NOW at the Polytecnic University of Valencia in Spain. The meeting was organized by Dr. Juan F. Asturiano and his colleagues. The meeting was attended by colleagues representing various institutions in Germany, Italy, Denmark, the Netherlands, France, Spain and Hungary. We are looking forward to a successful application and keep our fingers crossed.

Attendees of the project meeting

Bilateral project with Serbian colleagues

In the period between 20^th and 25^th May we hosted dr Nada Tokodi and dr Damjana Drobac from the Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad (Serbia). They were in Gödöllő as a part of the bilateral project TÉT_16-1-2016-0176 (NKFI Hivatal) "Invasive and blooming cyanobacteria in Serbian and Hungarian waters". The main activity during their stay was field trip to two lakes which we use for examination of presence of cyanobacteria in water and cyanotoxins in water and fish tissue. We also collected fish various tissues in order to analyze them for potential histopathological effects. Beside this, Nada and Damjana joined us in our current experiments regarding gonad cryopreservation and visited our zebrafish facilities. Since we spent such a good time together, we are looking forward to hosting them again in July.

We would like to thank our dear colleagues Dr. Árpád Ferincz,  Dr. Ádám Staszny, Vera Juhás and Bence Ivanovics for their precious help and collaboration.

Start of fishing with Árpi and Ádám

Vera collecting water samples

Vera checking physicochemical properties of the water

Sampling of the fish tissue

Whole team for field work (except Nada who took the picture)

Team work

Posing during work (special friend in the background)

Special friend smelling food

With Bence in zebrafish facilities

With Bence in zebrafish facilities

Bence showing zebrafish spawning

With Zoran during cryopreservation experiment

With Zoran and Ilija during preparation for tissue dissociation

Ilija i Damjana during tissue vitrification experiment

Zoran and Damjana during checking the viability of cells

Nada and Damjana during presentation of their previous work to our colleagues from the Department

Nada and Damjana during presentation of their previous work to our colleagues from the Department

We also had some discussion after their presentation